Modifying Enzymes
New England Biolabs, Inc. Lambda Protein Phosphatase – 20000 units
Lambda Protein Phosphatase is a Mn2+-dependent protein phosphatase with activity towards phosphorylated serine, threonine and tyrosine residues.
Apexbio Technology LLC EZ Cap CM-EGFP Probe mRNA (m1Ψ) 5x1mg (1mg/mL)
CM-EGFP mRNA encodes a fusion protein comprised of a cell membrane (CM) targeting signal sequence and enhanced green fluorescent protein (EGFP) By fusing EGFP with a membrane localization signal this probe enables real-time visualization of dynamic changes occurring at the cell membrane of living cells particularly beneficial for research involving cell membrane-related processes such as signal transduction membrane protein interactions and alterations in membrane structure EZ Cap CM-EGFP Probe mRNA (m1 ) is provided at 1 mg/mL It is specifically designed for cell membrane-specific expression of a green fluorescent protein label This mRNA is synthesized through co-transcriptional capping resulting in a Cap 1 structure The incorporation of m1 modifications poly(A) tail significantly enhances mRNA stability and lifetime both in vitro and in vivo
Carnabio Usa Inc TAOK2/10UG/CATALYTIC
TAOK2, Catalytic domain, Wild type, Amino Acid 1-319, NP_004774.1, Expressed in Insect (sf21); 10microg
New England Biolabs, Inc. NcoI-HF – 1000 units
A high fidelity restriction endonuclease that recognizes the sequence C^CATG_G. High Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity. HF enzymes are all Time-Saver qualified and can therefore cut substrate DNA in 5-15 with the flexibility to digest overnight without degradation to DNA. Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design. NEB extensively performs quality controls on all standard and high-fidelity (HF) restriction enzymes.
Sigma Aldrich Fine Chemicals Biosciences Proteinase K from Tritirachium album lyophilized powder, >=30 units/mg protein | 39450-01-6 | MFCD00132129 | 10MG
Proteinase K from Tritirachium album lyophilized powder, >=30 units/mg protein | 39450-01-6 | MFCD00132129 | 10MG
Carnabio Usa Inc QIK(SNF1LK2)/10UG/FULL-LENGTH
QIK(SNF1LK2), Full-length, Wild type, Amino Acid 1-926, NP_056006.1, Expressed in Insect (sf21); 10microg
Sigma Aldrich Fine Chemicals Biosciences β-Galactosidase from Escherichia coli | 9031-11-2 | MFCD00130623 | 1ku
β-Galactosidase from Escherichia coli | 9031-11-2 | MFCD00130623 | 1ku
Worthington Biochemical Corporation Elastase, Lyophilized, 25mg
Two times crystallized, (Code: ESL), supplied as a dialyzed, lyophilized powder. The enzyme should be 0.22 micron filtered after reconstitution and prior to use. Suitable for the isolation of Type II lung cells. Store at 2-8°C.
New England Biolabs, Inc. PspXI – 200 units
A restriction endonuclease that recognizes the sequence VC^TCGA_GB.
ABclonal Technology Endonuclease VIII
Endonuclease VIII originates from E.coli and possesses both N-glycosylase and AP-endonuclease activity. The N-glycosylase activity releases damaged pyrimidines from double-stranded DNA, generating an apurinic (AP site). The AP-endonuclease the phosphodiester bond at the AP site, forming 3’-P and 5’-P ends. Damaged bases recognized and removed by Endonuclease VIII include: urea, 5, 6-dihydroxythymine, thymine glycol, 5-hydroxy-5-methylhyd antoin, uracil glycol, 6-hydroxy-5, 6-dihydrothymine and methylhydroxy- methyluracil.
Worthington Biochemical Corporation VENOM PHOSPHODIESTERASE I
Purified by the method of Williams, Sung and Laskowski, JBC, 236, 1130 (1961). Further treated to inactivate contaminating 5'-nucleotidase activity according to Sulkowski and Laskowski: BBA, 240, 443 (1961). Lyophilized in vials.